Evident (former Olmpus) Laser scanning Microscope

Lasers: 375 nm, 405 nm, 445nm, 488nm, 514nm, 561nm, 640nm.

Objectives: 1,25x: air, 4x; air, 10x; air, 20x; air, 60x; immersion oil, 100x; silicon oil

• High Sensitivity Multi-Channel Imaging
• Macro to Micro Imaging and Super Resolution
• Increase Productivity with High-Speed Imaging
• Accurate Time-Lapse Imaging
• Superior Objectives

Introduction:

Leica™ Widefield Microhub

• 4 Objectives 1.6x, 10x, 20x, 63x
• 4 LEDs 365 nm, 470 nm, 555 nm, 625 nm
• High resolution Imaging CMOS camera
• Live cell imaging
• Long-term Time Lapse acquisition
• CO2 incubator
• Built-in dark room
• Sample finder
• OneTouch-Auto-Illumination
• AI based analysis
• Integrated Modulation Contrast (IMC)
• Reusable AI models and project parameters

Introduction:

Zellkraftwerk™ multiplexed imaging Technology

• Highly Multiplexed Tissue Imaging (HMTI) based on cyclic immunofluorescence
• More than 30 markers per cell for cryopreserved and FFPE tissues and more than 100 markers per cell for single cell suspensions
• Antibody stainings can further be combined with mRNA in-situ hybridization (FISH)
• High dynamic range (HDR) imaging technology and true background subtraction for superior image quality
• Microscope performs all steps from sample readjustment, focusing, and image acquisition automatically.
• Pipeline for automated signal quantification developed by us can be used for data analysis

Jarosch et al.: Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry

Protocol:

Introduction:

Leica™ Slide Scanner

Fast, flexible and reliable, it is easy-to-use and consistently delivers high resolution, high quality images.

• Load and capture images of an entire carousel in less than 8 hours
• Sustained high throughput rate of 50 slides/hr with a 20x Objective
• Autoloader with 400-slide capacity
• Smaller file size and faster scanning with skip blank stripes technology
• Horizontal position minimizes risk of coverslips falling off
• Better first time success rates with fewer rescans
• Quiet, with minimal movement of slides in loader

Introduction:

Invitrogen™

EVOS FL Auto 2

• Speed: scan a 96-well plate in 3 fluorescent channels in less than 5 minutes
• Time-lapse live-cell imaging: option for precise control of temperature, humidity, and gases for normoxic or hypoxic conditions allows a wide range of biological studies under physiological conditions with an onstage incubator
• Area view: move rapidly and seamlessly between low-magnification, single-field mode and high-magnification scan mode to easily define and capture the area of interest
• Automation: time-saving features such as autofocus, rapid stage movement, and automated routines help reduce time to complete experiments, allowing high throughput, high data quality, and improved experimental reproducibility
• Data analysis: advanced software package for quantitative and statistical analysis

Introduction:

Perkin Elmer™ In Vivo Imaging System

• High-sensitivity in vivo imaging of fluorescence and bioluminescence
• High throughput with 23 cm field of view
• High resolution (to 20 microns) with 3.9 cm field of view
• Twenty eight high efficiency filters spanning 430 – 850 nm
• Supports spectral unmixing applications
• Ideal for distinguishing multiple bioluminescent and fluorescent reporters
• Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
• 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence
• Ability import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.
• NIST traceable absolute calibrations

Introduction: